Difference between revisions of "Part:BBa K4156126"

Revision as of 14:37, 11 October 2022

HlpA with INP tag

HlpA is a binding protein from Streptococcus gallolyticus that binds to acetyl heparan sulfate glycoprotein (HSPG) on the tumor surface (specifically to syndecan 1, which is upregulated during carcinogenesis). HlpA has been shown to enhance the ability of engineered bacteria to penetrate colorectal tumor cells.

Usage and Biology

Characterization

HlpA is a new basic part of LZU-CHINA 2022, which we designed to facilitate better adhesion of engineered strains to cancer cells. In order to verify the function of this component, we characterized it. To demonstrate that HlpA enables better bacterial adhesion to cells, we constructed HlpA with mRFP to characterize HlpA. And characterization details can be found at BBa_K4156128

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 330
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 72
Illegal NgoMIV site found at 154
Illegal NgoMIV site found at 405
Illegal AgeI site found at 733
1000
COMPATIBLE WITH RFC[1000]

@@ Line 10: / Line 10: @@
 ===Characterization===
+HlpA is a new basic part of LZU-CHINA 2022, which we designed to facilitate better adhesion of engineered strains to cancer cells. In order to verify the function of this component, we characterized it.
 To demonstrate that HlpA enables better bacterial adhesion to cells, we constructed HlpA with mRFP to characterize HlpA. And characterization details can be found at<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156128"> BBa_K4156128 </a></html>