Difference between revisions of "Part:BBa K4367010:Design"
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===Source=== | ===Source=== | ||
| − | The protease | + | The protease originates from the Tobacco Etch Virus. |
===References=== | ===References=== | ||
Latest revision as of 11:49, 12 October 2022
Tobacco Etch Virus Protease (TEVp)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 799
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 14
Illegal BsaI.rc site found at 806
Design Notes
It's important to split at the right amino acids, between 118 and 119. The choice of TEVp was for its high specificity and the possibility of splitting it into two halves that could complement each other to regain catalytic function.
Source
The protease originates from the Tobacco Etch Virus.