Difference between revisions of "Part:BBa K4376001"
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We amplified two bpsA fragments then transformed recombinant vector into DH5α and Corynebacterium glutamicum.
 
We amplified two bpsA fragments then transformed recombinant vector into DH5α and Corynebacterium glutamicum.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:33, 11 October 2022


Produce indigoidine

Its encoding protein is blue-pigment indigoidine synthetase, which carries strong fluxes toward L-glutamate, a precursor of indigoidine. In our project, we sue it to produce indigoidine.

We amplified two bpsA fragments then transformed recombinant vector into DH5α and Corynebacterium glutamicum.

Engineering-1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3887
    Illegal XhoI site found at 3040
    Illegal XhoI site found at 3739
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 286
    Illegal NgoMIV site found at 693
    Illegal AgeI site found at 1951
    Illegal AgeI site found at 2004
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 229
    Illegal BsaI site found at 1540
    Illegal BsaI site found at 2254
    Illegal BsaI.rc site found at 358
    Illegal BsaI.rc site found at 3544
    Illegal SapI.rc site found at 1792