Difference between revisions of "Part:BBa K4461012"
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<a href="https://parts.igem.org/Part:BBa_K1051207">BBa_K1051207</a> | <a href="https://parts.igem.org/Part:BBa_K1051207">BBa_K1051207</a> | ||
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) with EcoRI and HindIII cutting sites on its prefix and suffix. Therefore, we can insert tag into our construct ( | ) with EcoRI and HindIII cutting sites on its prefix and suffix. Therefore, we can insert tag into our construct ( | ||
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Latest revision as of 13:19, 11 October 2022
mCerulean+tag fusion protein
We synthesized a sequence of tag ( BBa_K1051207 ) with EcoRI and HindIII cutting sites on its prefix and suffix. Therefore, we can insert tag into our construct ( BBa_K4461011 ) by restriction enzyme digestion.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 730
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 730
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 730
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 730
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 730
- 1000COMPATIBLE WITH RFC[1000]