Difference between revisions of "Part:BBa K4165149"
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===Usage and Biology=== | ===Usage and Biology=== | ||
the expression of serine protease HtrA1 which is an ATP-independent protease that can work extra- and intracellularly, It has been used in this project to target and degrade both tau and Aβ proteins which are both considered main causes of Alzheimer’s Disease pathogenesis. | the expression of serine protease HtrA1 which is an ATP-independent protease that can work extra- and intracellularly, It has been used in this project to target and degrade both tau and Aβ proteins which are both considered main causes of Alzheimer’s Disease pathogenesis. | ||
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===<span class='h3bb'>Sequence and Features</span>=== | ===<span class='h3bb'>Sequence and Features</span>=== | ||
Latest revision as of 13:44, 13 October 2022
T7 promoter-6x His tag-HtrA1-T7 terminator
This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pET22b RBS (BBa_K2924053), 6x His-tag (BBa_K4165020), HtrA1 (BBa_K4165004), and T7 terminator (BBa_K731721),The His tag was attached to the HTRA1 coding sequence to serve in the purification using NI-NTA column.
Usage and Biology
the expression of serine protease HtrA1 which is an ATP-independent protease that can work extra- and intracellularly, It has been used in this project to target and degrade both tau and Aβ proteins which are both considered main causes of Alzheimer’s Disease pathogenesis.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 181
- 1000COMPATIBLE WITH RFC[1000]
Dry Lab
Mathematical modeling
Transcription rate and translation rate under T7 promotor
the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab.
Figure 1. this figure shows the results from the transcription and translation code showing the
variation of mRNA and protein concentrations with time .