Fig. 1: WB result without ProQC system. (from left to right: Cas12b protein, LysPBC5 protein, LysPBC5 protein, cell lysate flow through of Cas12b protein)
Fig. 1: WB result without ProQC system. (from left to right: Cas12b protein, LysPBC5 protein, LysPBC5 protein, cell lysate flow through of Cas12b protein)
Revision as of 13:00, 11 October 2022
Lys+Quality control
Introduction
The protein quality control system (ProQC system) is a system that could enhance the integrity of the protein product by preventing the translation of truncated mRNA. It involves a prefix (switch) and a suffix (trigger) on the two sides of the ORF. Our team applied it to LysPBC5 expression and improved upon BBa_K653003 to get BBa_K4204019 as a universal expression model for the ProQC system for future iGEM teams to use (in which the GFP could be replaced by other parts). This page compares the western blotting result with and without the ProQC system to prove its effectiveness. Since we need to extract protein and do western blotting to verify the effect, His-tagged LysPBC5 is used as an example protein for efficiency.