Difference between revisions of "Part:BBa K4165168"
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===Usage and Biology=== | ===Usage and Biology=== | ||
Amyloid β Protein Fragment 1-42 results from the amyloidogenic degradation of Amyloid beta precursor and forms toxic plaques inside the brain leading to the development of Alzheimer's Disease, this protein which we target to degrade in our project. | Amyloid β Protein Fragment 1-42 results from the amyloidogenic degradation of Amyloid beta precursor and forms toxic plaques inside the brain leading to the development of Alzheimer's Disease, this protein which we target to degrade in our project. | ||
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===<span class='h3bb'>Sequence and Features</span>=== | ===<span class='h3bb'>Sequence and Features</span>=== | ||
Latest revision as of 13:44, 13 October 2022
Status: 500
Content-type: text/html
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This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), RBS 6 (BBa_K4165095), 6x His-tag (BBa_K4165020), amyloid beta (BBa_K4165005), and T7 terminator (BBa_K731721), The His tag was attached to the Amyloid beta coding sequence to serve in the purification using NI-NTA column.
Usage and Biology
Amyloid β Protein Fragment 1-42 results from the amyloidogenic degradation of Amyloid beta precursor and forms toxic plaques inside the brain leading to the development of Alzheimer's Disease, this protein which we target to degrade in our project.
Sequence and Features
Status: 500 Content-type: text/html
Software error:
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 230, near ""<BR>$group_name, $year, $team_name, $team_digits, $range_name, $range_prefix")" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
Dry Lab
Mathematical modeling
Transcription rate and translation rate under T7 promotor
the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab.
Figure 1. this figure shows the results from the transcription and translation code showing the
variation of mRNA and protein concentrations with time.