Difference between revisions of "Part:BBa K4146103"
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The main caffeine biosynthetic pathway is a sequence consisting of xanthosine→7-methylxanthosine→7-methylxanthine→theobromine→caffeine. Genes encoding N-methyltransferases involved in three of these four reactions have been isolated and the molecular structure of N-methyltransferases investigated. | The main caffeine biosynthetic pathway is a sequence consisting of xanthosine→7-methylxanthosine→7-methylxanthine→theobromine→caffeine. Genes encoding N-methyltransferases involved in three of these four reactions have been isolated and the molecular structure of N-methyltransferases investigated. | ||
| + | This gene can be found in E. coli Nissle 1917 | ||
| + | This gene is present in part<a href = "https://parts.igem.org/Part:BBa_K4146106"> BBa_K4146106</a> | ||
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Revision as of 15:20, 11 October 2022
TcCS2
TcCS2 is used for caffeine synthesis. TcCS2 from C. sinensis has a higher propensity to recognize 3-mX and can convert 3-mX to theobromine. The main caffeine biosynthetic pathway is a sequence consisting of xanthosine→7-methylxanthosine→7-methylxanthine→theobromine→caffeine. Genes encoding N-methyltransferases involved in three of these four reactions have been isolated and the molecular structure of N-methyltransferases investigated.
This gene can be found in E. coli Nissle 1917 This gene is present in part<a href = "https://parts.igem.org/Part:BBa_K4146106"> BBa_K4146106</a>
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 549
- 1000COMPATIBLE WITH RFC[1000]