Difference between revisions of "Part:BBa K243000:Design"

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===References===
 
===References===
  
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Mary C. Looneya, Laurie S. Morana, William E. Jacka, George R. Feeherya, Jack S. Bennera, Barton E. Slatkoa and Geoffrey G. Wilson;(1989)
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Mary C. Looneya, Laurie S. Morana, William E. Jacka, George R. Feeherya, Jack S. Bennera, Barton E. Slatkoa and Geoffrey G. Wilson;(1989)<br> Nucleotide sequence of the FokI restriction-modification system: separate strand-specificity domains in the methyltransferase ''Gene'' Vol.80 Issue:2 Pages:193-208
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Nucleotide sequence of the FokI restriction-modification system: separate strand-specificity domains in the methyltransferase ''Gene'' Vol.80 Issue:2 Pages:193-208
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Revision as of 13:49, 8 October 2009

Protein domain (active) of the restriction endonuclease FokI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 487


Design Notes

Modifications of the vector (catalytic active heterodimer) -heterodimeric aminio acids 

  * switch Glutamate 490/310-312 to Lysin (GAA->AAA)

  * switch isoleucin 538/454-456 to Lysin (ATC->AAA)


Source

extract coding region of Fok from the restriction-modification genes of the chromosomal DNA of Flavobacterium okeanokoites. Part synthesized by Mr.Gene


References

Mary C. Looneya, Laurie S. Morana, William E. Jacka, George R. Feeherya, Jack S. Bennera, Barton E. Slatkoa and Geoffrey G. Wilson;(1989)
Nucleotide sequence of the FokI restriction-modification system: separate strand-specificity domains in the methyltransferase Gene Vol.80 Issue:2 Pages:193-208