Difference between revisions of "Part:BBa K4229033"
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We had this BioBrick together with BBa_K4229034 on our Plasmid, which was transformed into BL21(DE3) E.Coli.  
 
We had this BioBrick together with BBa_K4229034 on our Plasmid, which was transformed into BL21(DE3) E.Coli.  
 
We successfully produced Indigo and Indirubin with our transformed bacteria. The production of the compounds was shown visibly by the colour change of the culture.
 
We successfully produced Indigo and Indirubin with our transformed bacteria. The production of the compounds was shown visibly by the colour change of the culture.
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[[File:IndigoLaborBild.jpg|600px|thumb|left|A: Left Erlenmeyer flasks with 200 mL culture after Indigo-production, right Erlenmeyer flasks with 200 mL culture after Indirubin-production; B: Pellet of 1.5 mL bacterial culture after Indigo-production in 200 mL culture followed by centrifugation; C: Pellet of 1.5 mL bacterial culture after Indirubin-production in 200 mL culture followed by centrifugation. For Indirubin production, L-Cysteine was added to shift the reaction. ]]
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[[File:IndigoLaborBild.jpg|500px|thumb|left|A: Left Erlenmeyer flasks with 200 mL culture after Indigo-production, right Erlenmeyer flasks with 200 mL culture after Indirubin-production; B: Pellet of 1.5 mL bacterial culture after Indigo-production in 200 mL culture followed by centrifugation; C: Pellet of 1.5 mL bacterial culture after Indirubin-production in 200 mL culture followed by centrifugation. For Indirubin production, L-Cysteine was added to shift the reaction. ]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:02, 11 October 2022


TnaA fused with SnoopCatcher N-terminal and Fre under T7v promoter and LacOperator

We had this BioBrick together with BBa_K4229034 on our Plasmid, which was transformed into BL21(DE3) E.Coli. We successfully produced Indigo and Indirubin with our transformed bacteria. The production of the compounds was shown visibly by the colour change of the culture.

A: Left Erlenmeyer flasks with 200 mL culture after Indigo-production, right Erlenmeyer flasks with 200 mL culture after Indirubin-production; B: Pellet of 1.5 mL bacterial culture after Indigo-production in 200 mL culture followed by centrifugation; C: Pellet of 1.5 mL bacterial culture after Indirubin-production in 200 mL culture followed by centrifugation. For Indirubin production, L-Cysteine was added to shift the reaction.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1064
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1064
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1064
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1064
    Illegal AgeI site found at 229
    Illegal AgeI site found at 315
    Illegal AgeI site found at 1687
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2224
    Illegal SapI.rc site found at 2344