Difference between revisions of "Part:BBa K4156083"
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<partinfo>BBa_K4156083 short</partinfo> | <partinfo>BBa_K4156083 short</partinfo> | ||
| − | φ174E is a kind of phage cleavage gene | + | φ174E is a kind of phage cleavage gene. Expression of this gene triggers bacterial lysis and death <sup>[1-3]</sup>, thereby releasing therapeutic factors stored in the bacteria. |
<!-- Add more about the biology of this part here --> | <!-- Add more about the biology of this part here --> | ||
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We used φ174E in the E. coli lysis experiment with validation of therapeutic efficacy, and characterization could be seen at <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156100"> BBa_K4156100 </a></html> ; <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156105"> BBa_K4156105 </a></html> ； <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156109"> BBa_K4156109 </a></html>. | We used φ174E in the E. coli lysis experiment with validation of therapeutic efficacy, and characterization could be seen at <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156100"> BBa_K4156100 </a></html> ; <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156105"> BBa_K4156105 </a></html> ； <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156109"> BBa_K4156109 </a></html>. | ||
| + | ===References=== | ||
| + | <i> | ||
| + | 1 Prindle A, Samayoa P, Razinkov I, Danino T, Tsimring LS, Hasty J. A sensing array of radically coupled genetic 'biopixels'. Nature. Dec 18 2011;481(7379):39-44. doi:10.1038/nature10722 | ||
| + | 2 Young KD, Young R. Lytic action of cloned phi X174 gene E. J Virol. Dec 1982;44(3):993-1002. doi:10.1128/jvi.44.3.993-1002.1982 | ||
| + | 3 Marguet P, Tanouchi Y, Spitz E, Smith C, You L. Oscillations by minimal bacterial suicide circuits reveal hidden facets of host-circuit physiology. PLoS One. Jul 30 2010;5(7):e11909. doi:10.1371/journal.pone.0011909 | ||
| + | </i> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 11:47, 11 October 2022
φ174E
φ174E is a kind of phage cleavage gene. Expression of this gene triggers bacterial lysis and death [1-3], thereby releasing therapeutic factors stored in the bacteria.
Usage and Biology
φ174E induces bacteria lysis in our constructed effector strains, thereby releasing intracellularly expressed therapeutic proteins.
Characterization
We used φ174E in the E. coli lysis experiment with validation of therapeutic efficacy, and characterization could be seen at BBa_K4156100 ; BBa_K4156105 ； BBa_K4156109 .
References
1 Prindle A, Samayoa P, Razinkov I, Danino T, Tsimring LS, Hasty J. A sensing array of radically coupled genetic 'biopixels'. Nature. Dec 18 2011;481(7379):39-44. doi:10.1038/nature10722
2 Young KD, Young R. Lytic action of cloned phi X174 gene E. J Virol. Dec 1982;44(3):993-1002. doi:10.1128/jvi.44.3.993-1002.1982
3 Marguet P, Tanouchi Y, Spitz E, Smith C, You L. Oscillations by minimal bacterial suicide circuits reveal hidden facets of host-circuit physiology. PLoS One. Jul 30 2010;5(7):e11909. doi:10.1371/journal.pone.0011909
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]