Difference between revisions of "Part:BBa K4165139"
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                            Figure 1: Predicted 3D structure of Synthetic peptide TLKIVW.
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                                Figure 1: Predicted 3D structure of Synthetic peptide TLKIVW.
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===References===
 
===References===

Latest revision as of 11:36, 11 October 2022


TLKIVW Peptide

A synthetic peptide that is used for targeting misfolded tau protein (BBa_K4165009) as it binds to PHF6 of tau fibrils.


Usage and Biology

TLKIVW is a hexapeptide designed using Rosetta software and obtained by phage display to bind to VQIVYK (BBa_K4165204) steric zipper motif of tau fibers, which is from the main reasons of the Alzheimer’s disease and other dementia related diseases, this peptide can also prevent further accumulation of tau fibrils.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Dry Lab

Modeling

TLKIVW was modeled by AlphaFold2 and Apptest and the best model was obtained from Apptest ranking 5 out of 6 according to our quality assessment code.


cbeta_deviations molprobity ramachandran_favored ramachandran_outliers Qmean_4 Qmean_6
0 2.11 100 0 -1.97474 -0.69185 


                                Figure 1: Predicted 3D structure of Synthetic peptide TLKIVW.

References

1. Sievers, S. A., Karanicolas, J., Chang, H. W., Zhao, A., Jiang, L., Zirafi, O., ... & Eisenberg, D. (2011). Structure-based design of non-natural amino-acid inhibitors of amyloid fibril formation. Nature, 475(7354), 96-100.