Difference between revisions of "Part:BBa K4229016"

Revision as of 11:43, 11 October 2022

RBS-snoopCatcherTnaA-RBS-FRE

These are the genes we put inside MCS1 of our vector pCDF-Duet-1. The first gene is the fusion protein of TnaA with the snoopcatcher(BBa_K4229013) on the C-terminal, the second one being Fre(BBa_K4229001). It was decided to put the enzymes with the catcher at the beginning of each MCS as those are our most important proteins, which we wanted to have produced the most. These two genes build together with the Biobrick BBa_K42218 all enzymes of the indigo/indirubin pathway. The production was tested under the T7 promotor and LacI operator in those two Biobricks: BBa_K4229033 and BBa_K4229034)

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 990
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 990
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 990
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 990
Illegal AgeI site found at 155
Illegal AgeI site found at 241
Illegal AgeI site found at 1613
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 2150
Illegal SapI.rc site found at 2270

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4229016 short</partinfo>
-These are the genes we put inside MCS1 of our vector pCDF-Duet-1. The first gene is the fusion protein of TnaA with the snoopcatcher(BBa_K4229011) on the C-terminal, the second one being Fre(BBa_K4229001). It was decided to put the enzymes with the catcher at the beginning of each MCS as those are our most important proteins, which we wanted to have produced the most. These two genes build together with the Biobrick BBa_K42218 all enzymes of the indigo/indirubin pathway. The production was tested under the T7 promotor and LacI operator in those two Biobricks: BBa_K4229033 and BBa_K4229034)
+These are the genes we put inside MCS1 of our vector pCDF-Duet-1. The first gene is the fusion protein of TnaA with the snoopcatcher(BBa_K4229013) on the C-terminal, the second one being Fre(BBa_K4229001). It was decided to put the enzymes with the catcher at the beginning of each MCS as those are our most important proteins, which we wanted to have produced the most. These two genes build together with the Biobrick BBa_K42218 all enzymes of the indigo/indirubin pathway. The production was tested under the T7 promotor and LacI operator in those two Biobricks: BBa_K4229033 and BBa_K4229034)
 [[File:Grafik indigo weier bg.png|900px|thumb|left|]]