Difference between revisions of "Part:BBa K4252001"
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==Result== | ==Result== | ||
| − | ===Results of pET-22b(+)-yjbB sequencing (primers: YjbB-2-5′, YjbB-2-3′) | + | ===Results of pET-22b(+)-yjbB sequencing (primers: YjbB-2-5′, YjbB-2-3′)=== |
<br> | <br> | ||
[[File: results-of-pet-22b-yjbB-sequencing.png|500px|thumb|center|Figure 1: results-of-pet-22b-yjbB-sequencing.]] | [[File: results-of-pet-22b-yjbB-sequencing.png|500px|thumb|center|Figure 1: results-of-pet-22b-yjbB-sequencing.]] | ||
Revision as of 10:17, 11 October 2022
phosphate exportation
As a phosphorus export element.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 426
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1174
Introduction
So far, only one type of P exporting protein, YjbB, has been reported in E. coli.The mechanism of phosphorus export by yjbB is still unclear. But based on experimental phenomena we choose YjbB as the phosphorus output element in our project.
Engineering
DNA fragments containing yjbB was amplified from E. coli MG1655 genomic DNA using the primers yjbB-5’/yjbB-3’.The PCR fragments were inserted into the Xbal/Xhol sites of pET-22b(+).
Result
Results of pET-22b(+)-yjbB sequencing (primers: YjbB-2-5′, YjbB-2-3′)
