Difference between revisions of "Part:BBa K4365001"
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Turbo Red Fluorescent Protein (turboRFP) is an orange fluorescent protein isolated from <i>Entacmaea quadricolor</i> with excitation and emission maxima 553 and 574 respectively. It could be used as a selection marker or used for protein detection if fused with another protein.
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Turbo Red Fluorescent Protein (turboRFP) is an orange fluorescent protein isolated from <i>Entacmaea quadricolor</i> with excitation and emission maxima 553 and 574 respectively. It could be used as a selection marker or for protein detection if fused with another protein.
  
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4365001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4365001 SequenceAndFeatures</partinfo>
  
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===Usage and Biology===
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TurboRFP is a red-orange fluorescent protein derived from sea anemone ''Entacmaea quadricolor'' <ref name="ameno">Merzlyak, E., Goedhart, J., Shcherbo, D. et al. Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods 4, 555–557 (2007). https://doi.org/10.1038/nmeth1062</ref>. Possessing prolonged fluorescence lifetime and high pH-stability.and high pH stability, TurboRFP is more than twice brighter than DsRed2. Fast TurboRFP maturation makes it clearly detectable in mammalian cells as early as within 8-10 hrs after transfection <ref name="ameno" />.
  
 
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Revision as of 14:32, 11 October 2022


turboRFP

Turbo Red Fluorescent Protein (turboRFP) is an orange fluorescent protein isolated from Entacmaea quadricolor with excitation and emission maxima 553 and 574 respectively. It could be used as a selection marker or for protein detection if fused with another protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

TurboRFP is a red-orange fluorescent protein derived from sea anemone Entacmaea quadricolor [1]. Possessing prolonged fluorescence lifetime and high pH-stability.and high pH stability, TurboRFP is more than twice brighter than DsRed2. Fast TurboRFP maturation makes it clearly detectable in mammalian cells as early as within 8-10 hrs after transfection [1].

  1. ↑ 1.0 1.1 Merzlyak, E., Goedhart, J., Shcherbo, D. et al. Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods 4, 555–557 (2007). https://doi.org/10.1038/nmeth1062