Difference between revisions of "Part:BBa K4165175"
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===WetLab Results===
 
===WetLab Results===
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<p style=" font-weight: bold; font-size:14px;"> Transformation of His Tau in BL-21 using pGS-21a vector </p>
 
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/his-tau-pgs.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/his-tau-pgs.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
</html>
 
</html>
 
                                     Figure 2. Transformed plate of His Tau + pGS-21a
 
                                     Figure 2. Transformed plate of His Tau + pGS-21a
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<p style=" font-weight: bold; font-size:14px;"> Transformation of His Tau in DH-5 alpha using pJET vector </p>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/his-tau-pjet.jpg" style="margin-left:200px;" alt="" width="500" /></p>
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                                    Figure 3. Transformed plate of His Tau + pJET
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<!-- Uncomment this to enable Functional Parameter display  
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K4165175 parameters</partinfo>
 
<partinfo>BBa_K4165175 parameters</partinfo>
 
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Revision as of 11:25, 11 October 2022


Biobrick His - Tau

This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pGS-21a RBS (BBa_K4165016), 6x His-tag (BBa_K4165020), Tau (BBa_K4165009), and T7 terminator (BBa_K731721).

Source

Synthesized

Usage and Biology

In the AD brain, tau Hyperphosphorylation is considered the main cause of AD progression, it may alter the protein's shape and charge, which in turn causes the microtubule-binding domain to become exposed and allow tau to self-assemble and form oligomers characterized to be a neurofibrillary tangle. According to several studies, the polymerized tau (neurofibrillary tangles) is inert since it does not bind to tubulin or encourage its assembly into microtubules, the His Tag for the purification of the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 169
    Illegal AgeI site found at 355
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 333
    Illegal BsaI site found at 1245
    Illegal BsaI.rc site found at 45
    Illegal SapI.rc site found at 519

Dry Lab

Modeling

Mathematical modeling

Transcription rate and translation rate under T7 promotor

the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab. 


                 Figure 1. this figure shows the results from the transcription and translation code showing 
                the variation of mRNA and protein concentrations with time compared with the wet lab results.

WetLab Results

Transformation of His Tau in BL-21 using pGS-21a vector 

                                    Figure 2. Transformed plate of His Tau + pGS-21a

Transformation of His Tau in DH-5 alpha using pJET vector 

                                   Figure 3. Transformed plate of His Tau + pJET