Difference between revisions of "Part:BBa K4414009"
| Line 7: | Line 7: | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | |||
The Tet repressor protein (TetR) family is a ubiquitous family of transcriptional regulators in a variety of bacteria, with more than 2000 members. The first TetR family identified in Escherichia coli. was Tn10, which controls the expression of genes encoding the tetracycline spillover pump necessary for drug resistance.(Ramos et al., 2005) In the absence of tetracycline, TetR binds to the tetracycline operon and inhibits the transcription of tetracycline spillover pump genes. When tetracycline enters the cell, tetracycline binds to TetR and changes its conformation, dissociating TetR from the tet operator (tetO) sequence, thus releasing inhibition. TetR-like transcriptional regulators contain a conserved helix-turn-helix DNA binding region (forming homodimers) and generally act as transcriptional repressors. (Das et al., 2016)Their function is to broadly regulate cellular activities (including drug spillover, antibiotic synthesis, amino acid metabolism, etc.) | The Tet repressor protein (TetR) family is a ubiquitous family of transcriptional regulators in a variety of bacteria, with more than 2000 members. The first TetR family identified in Escherichia coli. was Tn10, which controls the expression of genes encoding the tetracycline spillover pump necessary for drug resistance.(Ramos et al., 2005) In the absence of tetracycline, TetR binds to the tetracycline operon and inhibits the transcription of tetracycline spillover pump genes. When tetracycline enters the cell, tetracycline binds to TetR and changes its conformation, dissociating TetR from the tet operator (tetO) sequence, thus releasing inhibition. TetR-like transcriptional regulators contain a conserved helix-turn-helix DNA binding region (forming homodimers) and generally act as transcriptional repressors. (Das et al., 2016)Their function is to broadly regulate cellular activities (including drug spillover, antibiotic synthesis, amino acid metabolism, etc.) | ||
Revision as of 08:58, 11 October 2022
TetR
DNA binding domain
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
[1] Das, A. T., Tenenbaum, L., & Berkhout, B. (2016). Tet-On Systems For Doxycycline-inducible Gene Expression. Current Gene Therapy, 16(3), 156–167. https://doi.org/10.2174/1566523216666160524144041 [2] Ramos, J. L., Martínez-Bueno, M., Molina-Henares, A. J., Terán, W., Watanabe, K., Zhang, X., Gallegos, M. T., Brennan, R., & Tobes, R. (2005). The TetR family of transcriptional repressors. Microbiology and Molecular Biology Reviews: MMBR, 69(2), 326–356. https://doi.org/10.1128/MMBR.69.2.326-356.2005