Difference between revisions of "Part:BBa K4361112"

Revision as of 08:41, 11 October 2022

Blc operator SDM primer F

A site-directed mutagenesis primer, used to induce the deletion of adenine 20 in the Blc operator sequence (Part:BBa_K4361111) during PCR, in conjunction with the reverse primer Part:BBa_K4361113. As this deletion occurs in the DNA sequence which is recognized and bound by BlcR, it is expected to disrupt binding of BlcR. Thus the resulting mutated operator sequence (Part:BBa_K4361114) may act as a negative control when testing the binding properties of BlcR.