Difference between revisions of "Part:BBa K4361105"

Revision as of 12:48, 12 October 2022

pET-11a

A pET11a expression system in E. coli is popular because it combines a high protein yield with good regulation over the expression. The T7 RNA polymerase has a lacUV5 promoter that is Isopropyl 𝛃-D-1-thiogalactopyranoside (IPTG) inducible. With the addition of IPTG, the gene upstream of the T7 promoter can be transcribed. This part does not represent the full sequence of pET-11a, but rather the plasmid after digestion with BamHI as it allows for the insertion of a new DNA sequence at the cleavage site.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 316
Illegal XbaI site found at 5594
Illegal PstI site found at 1068
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 316
Illegal NheI site found at 5639
Illegal PstI site found at 1068
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 316
Illegal BglII site found at 5528
Illegal BamHI site found at 5672
23
INCOMPATIBLE WITH RFC[23]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 316
Illegal XbaI site found at 5594
Illegal PstI site found at 1068
25
INCOMPATIBLE WITH RFC[25]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 316
Illegal XbaI site found at 5594
Illegal PstI site found at 1068
Illegal NgoMIV site found at 3394
Illegal NgoMIV site found at 3748
Illegal NgoMIV site found at 3908
Illegal NgoMIV site found at 5496
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 1242
Illegal SapI.rc site found at 2324

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4361105 short</partinfo>
-Linearized sequence of the pET-11a expression plasmid. This part does not represent the full sequence of pET-11a, but rather the plasmid after digestion with BamHI as to allow for the insertion of a new DNA sequence at the cleavage site (see [[Part:BBa_K4361106]]). The T7 promoter on the plasmid allows for the transcription of the insert when the plasmid is added to a PURE reaction.
+A pET11a expression system in E. coli is popular because it combines a high protein yield with good regulation over the expression. The T7 RNA polymerase has a lacUV5 promoter that is Isopropyl 𝛃-D-1-thiogalactopyranoside (IPTG) inducible. With the addition of IPTG, the gene upstream of the T7 promoter can be transcribed. This part does not represent the full sequence of pET-11a, but rather the plasmid after digestion with BamHI as it allows for the insertion of a new DNA sequence at the cleavage site.
 <!-- Add more about the biology of this part here