Difference between revisions of "Part:BBa K4147013"

Latest revision as of 00:58, 12 October 2022

Down part of cassette for siRNA production

The lower part of the cassette is for the production of siRNA. It is composed of a reverse T7 promoter with an RBS (BBa_K4147014) and a T7 terminator (BBa_K864600).

In order to produce the siRNAs, this part is assembled with the upper part.

Usage and Biology

To produce the double-stranded siRNAs, an expression system has been designed and must be assembled. For this method of construct assembly for dsRNA expression, we were inspired by the method used by the Ecuador 2021 team. When analyzing the possible options to produce siRNA we found that it was possible to use assembly methods to make constructs that encode in both directions.

The figure below shows the assembly strategy that is planned to be used.

Assembly strategy for siRNA production

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 10: / Line 10: @@
 To produce the double-stranded siRNAs, an expression system has been designed and must be assembled.
-The assembly method is based on the one shown by the <b>iGEM Ecuador 2021 team</b>.
+For this method of construct assembly for dsRNA expression, we were inspired by the method used by the Ecuador 2021 team. When analyzing the possible options to produce siRNA we found that it was possible to use assembly methods to make constructs that encode in both directions.
 The figure below shows the assembly strategy that is planned to be used.