Difference between revisions of "Part:BBa K4193027:Design"

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===Design Notes===
 
===Design Notes===
 
Cytokinin isopentenyladenine (IP), as quorum sensing molecule, is the product of ATP catalyzed by AtlPT4. When the signal molecule IP reaches a certain concentration, it activates the SSRE promoter by triggering phosphorylation of a series of proteins such as AtCRE1 and Ypd1, and increases the expression of production gene controlled by SSRE promoter. To avoid non-specific HOG pathway induction through Ssk1, we expressed PTP2, the gene encoding an endogenous Hog1 protein phosphatase. In our experiment, to better characterize SSRE promoter and quorum sensing circuit, we put eGFP gene at the downstream of SSRE promoter.
 
Cytokinin isopentenyladenine (IP), as quorum sensing molecule, is the product of ATP catalyzed by AtlPT4. When the signal molecule IP reaches a certain concentration, it activates the SSRE promoter by triggering phosphorylation of a series of proteins such as AtCRE1 and Ypd1, and increases the expression of production gene controlled by SSRE promoter. To avoid non-specific HOG pathway induction through Ssk1, we expressed PTP2, the gene encoding an endogenous Hog1 protein phosphatase. In our experiment, to better characterize SSRE promoter and quorum sensing circuit, we put eGFP gene at the downstream of SSRE promoter.
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===Source===
 
===Source===

Revision as of 08:54, 12 October 2022


quorum sensing system (two parts)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 2520
    Illegal EcoRI site found at 4394
    Illegal EcoRI site found at 4719
    Illegal XbaI site found at 1618
    Illegal XbaI site found at 2077
    Illegal XbaI site found at 2161
    Illegal XbaI site found at 2524
    Illegal XbaI site found at 5680
    Illegal SpeI site found at 4231
    Illegal PstI site found at 4481
    Illegal PstI site found at 5180
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 2520
    Illegal EcoRI site found at 4394
    Illegal EcoRI site found at 4719
    Illegal SpeI site found at 4231
    Illegal PstI site found at 4481
    Illegal PstI site found at 5180
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 2520
    Illegal EcoRI site found at 4394
    Illegal EcoRI site found at 4719
    Illegal BglII site found at 166
    Illegal BglII site found at 1102
    Illegal BglII site found at 1576
    Illegal BglII site found at 3435
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 2520
    Illegal EcoRI site found at 4394
    Illegal EcoRI site found at 4719
    Illegal XbaI site found at 1618
    Illegal XbaI site found at 2077
    Illegal XbaI site found at 2161
    Illegal XbaI site found at 2524
    Illegal XbaI site found at 5680
    Illegal SpeI site found at 4231
    Illegal PstI site found at 4481
    Illegal PstI site found at 5180
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 2520
    Illegal EcoRI site found at 4394
    Illegal EcoRI site found at 4719
    Illegal XbaI site found at 1618
    Illegal XbaI site found at 2077
    Illegal XbaI site found at 2161
    Illegal XbaI site found at 2524
    Illegal XbaI site found at 5680
    Illegal SpeI site found at 4231
    Illegal PstI site found at 4481
    Illegal PstI site found at 5180
    Illegal NgoMIV site found at 5447
    Illegal NgoMIV site found at 5487
    Illegal NgoMIV site found at 5527
    Illegal NgoMIV site found at 5567
    Illegal AgeI site found at 5457
    Illegal AgeI site found at 5497
    Illegal AgeI site found at 5537
    Illegal AgeI site found at 5577
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cytokinin isopentenyladenine (IP), as quorum sensing molecule, is the product of ATP catalyzed by AtlPT4. When the signal molecule IP reaches a certain concentration, it activates the SSRE promoter by triggering phosphorylation of a series of proteins such as AtCRE1 and Ypd1, and increases the expression of production gene controlled by SSRE promoter. To avoid non-specific HOG pathway induction through Ssk1, we expressed PTP2, the gene encoding an endogenous Hog1 protein phosphatase. In our experiment, to better characterize SSRE promoter and quorum sensing circuit, we put eGFP gene at the downstream of SSRE promoter.

Source

synthesize from company

References

Yang, X., Liu, J., Zhang, J., Shen, Y., Qi, Q., Bao, X. and Hou, J. (2021). Quorum sensing-mediated protein degradation for dynamic metabolic pathway control in Saccharomyces cerevisiae. Metabolic Engineering, 64, pp.85–94. doi:10.1016/j.ymben.2021.01.010.