Difference between revisions of "Part:BBa K4361104"
 
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<partinfo>BBa_K4361104 short</partinfo>
 
<partinfo>BBa_K4361104 short</partinfo>
  
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Composite part consisting of [[Part:BBa_K4361102]], a linker sequence, and [[Part:BBa_K4361101]]. Because of the combination of purification tag and cleavage site, a protein with this combined part (e.g. [[Part:BBa_K4361103]]) can be purified from solution using the tag and subsequently separated from said tag through the cleavage site.
+
Composite part consisting of [[Part:BBa_K4361102]], a linker sequence, and [[Part:BBa_K4361101]].With the combination of the His-Tag and the TEV site a protein can first be purified with His-Tag purification technique and second be digested with TEV protease to cut off the His-TEV site.
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 12:33, 12 October 2022


6xHis-tag with TEV protease cleavage site

Composite part consisting of Part:BBa_K4361102, a linker sequence, and Part:BBa_K4361101.With the combination of the His-Tag and the TEV site a protein can first be purified with His-Tag purification technique and second be digested with TEV protease to cut off the His-TEV site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]