Difference between revisions of "Part:BBa K4361101"
 
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<partinfo>BBa_K4361101 short</partinfo>
 
<partinfo>BBa_K4361101 short</partinfo>
  
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Recognition sequence of the Tobacco Etch Virus (TEV) protease. This sequence is meant to be placed between a protein coding sequence and an isolation/purification tag (e.g. [[Part:BBa_K4361102]]). When a tagged protein ([[Part:BBa_K4361103]]) is expressed and subsequently exposed to the TEV protease, the tag is cleaved off of the protein to prevent the tag from affecting its functionality.
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Recognition sequence of the Tobacco Etch Virus (TEV) protease. This sequence is meant to be placed between a protein-coding sequence and a purification tag. TEV protease will digest the TEV site (ENLYFQ/G) from the protein to prevent the tag from affecting the protein activity.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 12:30, 12 October 2022


TEV protease cleavage site

Recognition sequence of the Tobacco Etch Virus (TEV) protease. This sequence is meant to be placed between a protein-coding sequence and a purification tag. TEV protease will digest the TEV site (ENLYFQ/G) from the protein to prevent the tag from affecting the protein activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]