Difference between revisions of "Part:BBa K4324300"
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<partinfo>BBa_K4324300 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4324300 SequenceAndFeatures</partinfo>
  
−
==Xylose Reductase (XYL1)==
+
==Xylose Reductase (BBa_K4324000)==
  
−
==Xylitol Dehydrogenase (XYL2)==
+
==Xylitol Dehydrogenase (BBa_K4324001)==
  
−
==Xylulose Kinase (xylB) ==
+
==Xylulose Kinase (BBa_K4324002) ==
  
−
==Phosphoketolase (XFP)==
+
==Phosphoketolase (BBa_K4324003)==
  
  

Revision as of 06:33, 11 October 2022


XR-XDH-XK-PK for E. coli

This part is a collection of all composite parts for xylose reductase (BBa_K4324000), xylitol dehydrogenase (BBa_K4324001), xylulose kinase (BBa_K4324002), and phosphoketolase (BBa_K4324003). This part is a collection of all composite parts within TheKingsSchool_AU_HS's project.

This part enables E. coli to express the XR-XDH pathway, further express XK, and express phosphoketolase (links xylulose-5-phosphate to glycolysis).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1649
    Illegal BglII site found at 4065
    Illegal BamHI site found at 4362
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5767
    Illegal AgeI site found at 2929
    Illegal AgeI site found at 3217
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1306
    Illegal SapI.rc site found at 747

Xylose Reductase (BBa_K4324000)

Xylitol Dehydrogenase (BBa_K4324001)

Xylulose Kinase (BBa_K4324002)

Phosphoketolase (BBa_K4324003)