Difference between revisions of "Part:BBa K4165240"

Revision as of 12:40, 11 October 2022

His-UBC

his biobrick consists of T7 promotor (BBa_K3633015), RBS 1 (BBa_K4165261), 6-His tag (BBa_K4165020), UBC (BBa_K4165014), T7 Terminator (BBa_K731721), The His tag was attached to the UBC coding sequence to serve in the purification using NI-NTA column.

source

Synthesized

Usage and Biology

the ubiquitin C which is essential in the degradation of misfolded proteins through the ubiquitin-proteasome cascade. It has been used in our project as signaling for the degradation of both tau and Aβ proteins which are considered the main causes of Alzheimer’s Disease pathogenesis.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 113
Illegal BamHI site found at 82
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Dry lab

Mathematical modeling

Transcription rate and translation rate under T7 promotor

the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab.

                Figure 1. this figure shows the results from the transcription and translation code showing the 
                                        the concentration of the protein with time.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4165240 short</partinfo>
-his biobrick consists of T7 promotor (BBa_K3633015), RBS (BBa_K4165016), 6-His tag (BBa_K4165020), UBC (BBa_K4165014), T7 Terminator (BBa_K731721), The His tag was attached to the UBC coding sequence to serve in the purification using NI-NTA column.
+his biobrick consists of T7 promotor (BBa_K3633015), RBS 1 (BBa_K4165261), 6-His tag (BBa_K4165020), UBC (BBa_K4165014), T7 Terminator (BBa_K731721), The His tag was attached to the UBC coding sequence to serve in the purification using NI-NTA column.
 ===source===