Difference between revisions of "Part:BBa K4444010"

 
Line 3: Line 3:
 
<partinfo>BBa_K4444010 short</partinfo>
 
<partinfo>BBa_K4444010 short</partinfo>
  
−
PhoCl Shed mRuby Backbone is a backbone modified from the RELEASE system. It contains only a tri-transmembrane domain; the ER retention motif, furin cut site, GFP,and HCV cut site were all removed.
+
PhoCl Shed mRuby is modified from the RELEASE system. It contains a mRuby and a tri-transmembrane domain; the ER retention motif, furin cut site, GFP, and HCV cut site were all removed.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 03:51, 12 October 2022


PhoCl Shed mRuby

PhoCl Shed mRuby is modified from the RELEASE system. It contains a mRuby and a tri-transmembrane domain; the ER retention motif, furin cut site, GFP, and HCV cut site were all removed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 769
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1654
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1288
    Illegal SapI.rc site found at 73