Difference between revisions of "Part:BBa K4335021"
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BBa_ K4335021 consists of a terminator, a cloning site, SpScaffold and a terminator | BBa_ K4335021 consists of a terminator, a cloning site, SpScaffold and a terminator | ||
| − | PCrU6.3 is a previously characterized RNA from chromosome 8 of Chlamydomonas reinhardtii [1] | + | PCrU6.3 is a previously characterized RNA from chromosome 8 of Chlamydomonas reinhardtii [1]<br> |
| + | <br> | ||
| + | Template of sgRNA. Use Golden Gate (BsaⅠ) to replace the template sequence with a ~20bp guide sequence. Add 'ACTT' on the 5'-terminal of your guide sequence and 'AAAC' on the reverse 5'-terminal.<br> | ||
| + | <br> | ||
| + | <figure> | ||
| + | <img src="https://static.igem.wiki/teams/4335/wiki/zhanghang-1.png" width="60%" style="float:center"> | ||
| + | <figcaption> | ||
| + | <p style="font-size:1rem">sgRNA | ||
| + | </p> | ||
| + | </figcaption> | ||
| + | </figure> | ||
<h2></h2> | <h2></h2> | ||
Revision as of 03:45, 11 October 2022
pCrU6.3+insert site+SpScaffold+polyT Term
BBa_ K4335021 consists of a terminator, a cloning site, SpScaffold and a terminator
PCrU6.3 is a previously characterized RNA from chromosome 8 of Chlamydomonas reinhardtii [1] sgRNA
Template of sgRNA. Use Golden Gate (BsaⅠ) to replace the template sequence with a ~20bp guide sequence. Add 'ACTT' on the 5'-terminal of your guide sequence and 'AAAC' on the reverse 5'-terminal.
