Difference between revisions of "Part:BBa K4182011:Design"

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Escherichia phage phiX174(from https://www.ncbi.nlm.nih.gov/gene/2546400)
 
Escherichia phage phiX174(from https://www.ncbi.nlm.nih.gov/gene/2546400)
  
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===References===
+
==References==
 +
1. Din, M.O., et al., Synchronized cycles of bacterial lysis for in vivo delivery. Nature, 2016. 536(7614): p. 81-85.
 +
 
 +
2. Saeidi, N., et al., Engineering microbes to sense and eradicate Pseudomonas aeruginosa, a human pathogen. Mol Syst Biol, 2011. 7: p. 521.
 +
 
 +
3. Restrepo-Pineda, S., et al., Thermoinducible expression system for producing recombinant proteins in Escherichia coli: advances and insights. FEMS Microbiol Rev, 2021. 45(6).
 +
 
 +
4. Aparicio, T., V. de Lorenzo, and E. Martínez-García, Improved Thermotolerance of Genome-Reduced Pseudomonas putida EM42 Enables Effective Functioning of the PL/cI857 System. Biotechnology Journal, 2019. 14(1): p. 1800483.

Revision as of 03:32, 11 October 2022


Temperature regulated suicide circuit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1018
    Illegal suffix found in sequence at 1446
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1018
    Illegal SpeI site found at 1447
    Illegal PstI site found at 1461
    Illegal NotI site found at 1024
    Illegal NotI site found at 1454
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1018
    Illegal XhoI site found at 2478
    Illegal XhoI site found at 3504
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1018
    Illegal suffix found in sequence at 1447
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1018
    Illegal XbaI site found at 1033
    Illegal SpeI site found at 1447
    Illegal PstI site found at 1461
  • 1000
    COMPATIBLE WITH RFC[1000]


Profile

Base Pairs

Design Notes

This gene has been optimized for E. coli

Source

Escherichia phage phiX174(from https://www.ncbi.nlm.nih.gov/gene/2546400)

References

1. Din, M.O., et al., Synchronized cycles of bacterial lysis for in vivo delivery. Nature, 2016. 536(7614): p. 81-85.

2. Saeidi, N., et al., Engineering microbes to sense and eradicate Pseudomonas aeruginosa, a human pathogen. Mol Syst Biol, 2011. 7: p. 521.

3. Restrepo-Pineda, S., et al., Thermoinducible expression system for producing recombinant proteins in Escherichia coli: advances and insights. FEMS Microbiol Rev, 2021. 45(6).

4. Aparicio, T., V. de Lorenzo, and E. Martínez-García, Improved Thermotolerance of Genome-Reduced Pseudomonas putida EM42 Enables Effective Functioning of the PL/cI857 System. Biotechnology Journal, 2019. 14(1): p. 1800483.