Difference between revisions of "Part:BBa K4169028"

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		+	__NOTOC__
		+	<partinfo>BBa_K4169028</partinfo>
		+	===HepT: a RNase like toxin===
		+	<p>HepT is a toxin that can be effectively inhibited the growth of bacteria.The HepT toxin could function as an RNase with a RX4-6H active motif and cleave mRNA to inhibit cell growth. Complete with HepT (HZAU-China 2021:BBa_K3733010) was used in this study.</p>
		+
		+	===Usage and Biology===
		+	<p>As an RNase enzyme, HepT can degrade mRNA at the transcriptional level, which ultimately leads to cell death
		+	</p>
		+
		+	===Sequence and Features===
		+	<partinfo>BBa_K4169006 SequenceAndFeatures</partinfo>
		+
		+
		+
		+	<h3>References</h3>
		+	<p>Hoynes-O'Connor A, Hinman K, Kirchner L, Moon TS. De novo design of heat-repressible RNA thermosensors in E. coli.<i> Nucleic Acids Res.</i> 2015 Jul 13;43(12):6166-79.</p>
		+
	__NOTOC__		__NOTOC__

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Revision as of 03:23, 11 October 2022

BBa_K4169028

HepT: a RNase like toxin

HepT is a toxin that can be effectively inhibited the growth of bacteria.The HepT toxin could function as an RNase with a RX4-6H active motif and cleave mRNA to inhibit cell growth. Complete with HepT (HZAU-China 2021:BBa_K3733010) was used in this study.

Usage and Biology

As an RNase enzyme, HepT can degrade mRNA at the transcriptional level, which ultimately leads to cell death

Sequence and Features

References

Hoynes-O'Connor A, Hinman K, Kirchner L, Moon TS. De novo design of heat-repressible RNA thermosensors in E. coli. Nucleic Acids Res. 2015 Jul 13;43(12):6166-79.

Pcyd: A promoter open in aerobic conditions

This is an aerobically initiated promoter that initiates transcription of subsequent sequences under microaerobic and aerobic conditions. The mechanism is based on the fact that Fnr takes over the promoter sequence under anaerobic conditions, competes with RNA polymerase for binding sites, and thus prevents the transcription of subsequent sequences. However, under microaerobic and aerobic conditions, Fnr protein does not bind to the sequence, and thus can continue the subsequent transcription.

Sequence and Features