Difference between revisions of "Part:BBa K4182011:Design"

 
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===Design Notes===
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==Profile==
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This gene has been optimized for E. coli
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===Base Pairs===
  
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===Design Notes===
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This gene has been optimized for E. coli
  
 
===Source===
 
===Source===

Revision as of 03:32, 11 October 2022


Temperature regulated suicide circuit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1018
    Illegal suffix found in sequence at 1446
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1018
    Illegal SpeI site found at 1447
    Illegal PstI site found at 1461
    Illegal NotI site found at 1024
    Illegal NotI site found at 1454
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1018
    Illegal XhoI site found at 2478
    Illegal XhoI site found at 3504
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1018
    Illegal suffix found in sequence at 1447
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1018
    Illegal XbaI site found at 1033
    Illegal SpeI site found at 1447
    Illegal PstI site found at 1461
  • 1000
    COMPATIBLE WITH RFC[1000]


Profile

Base Pairs

Design Notes

This gene has been optimized for E. coli

Source

Escherichia phage phiX174(from https://www.ncbi.nlm.nih.gov/gene/2546400)

References