Difference between revisions of "Part:BBa K4169028"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This is a promoter sequence that initiates transcription of subsequent gene sequences under aerobic conditions and is part of the operon structure of cydAB. Under anaerobic conditions, the Fnr protein will bind to a part of the promoter sequence, impeding RNA polymerase binding, playing a competitive role, and the downstream gene sequence cannot be transcribed. However, under microoxygen conditions, AcrA protein binds to this promoter and recruits RNA polymerase to bind to the promoter, thereby normally initiating downstream transcription. Under aerobic conditions, RNA polymerase normally binds to the promoter. So this is a promoter sequence that can be transcribed normally under aerobic conditions. | + | <p>This is a promoter sequence that initiates transcription of subsequent gene sequences under aerobic conditions and is part of the operon structure of cydAB. Under anaerobic conditions, the Fnr protein will bind to a part of the promoter sequence, impeding RNA polymerase binding, playing a competitive role, and the downstream gene sequence cannot be transcribed. However, under microoxygen conditions, AcrA protein binds to this promoter and recruits RNA polymerase to bind to the promoter, thereby normally initiating downstream transcription. Under aerobic conditions, RNA polymerase normally binds to the promoter. So this is a promoter sequence that can be transcribed normally under aerobic conditions. |
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Revision as of 03:16, 11 October 2022
Pcyd: A promoter open in aerobic conditions
This is an aerobically initiated promoter that initiates transcription of subsequent sequences under microaerobic and aerobic conditions. The mechanism is based on the fact that Fnr takes over the promoter sequence under anaerobic conditions, competes with RNA polymerase for binding sites, and thus prevents the transcription of subsequent sequences. However, under microaerobic and aerobic conditions, Fnr protein does not bind to the sequence, and thus can continue the subsequent transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]