Difference between revisions of "Part:BBa K4245010"

Revision as of 00:10, 11 October 2022

hsa-miR-150-5p

This part is the sequence for hsa-miR-150-5p, an miRNA isolated from Homo sapiens . This miRNA acts as a biomarker for coronary artery disease and is therefore potentially useful for the early detection of this condition. hsa-miR-150-5p is related to the inflammation process and is involved in the pathogenesis of coronary artery disease. Therefore, hsa-miR-150-5p is a means by which cardiologists can monitor the progression of the disease in patients (Zhang et al., 2015). However, hsa-miR-150-5p seems to be dysregulated, increased and decreased in concentration. Nevertheless, more research, with larger sample sets is needed to see if this biomarker is viable for CAD diagnosis (Kaur et al., 2020).

The Lambert_GA 2022 team developed a set of padlock probes to use the rolling circle amplification approaches for several miRNAs related to CAD. This miRNA is used as the basis for BBa_K4245104 and BBa_K4245111, the 3' arm for hsa-miR-150-5p and 5' arm for hsa-miR-150-5p, and as the target sequence for BBa_K4245204 the hsa-miR-150-5p RCA Padlock Probe.

When using rolling circle amplification (RCA), the miRNA binds to the padlock. A rolling circle product (RCP) is produced from BBa_K4245131 (Middle Sequence), which is then detected by the linear probes BBa_K4245130 (Fluorophore) and BBa_K4245132 (Quencher). When these parts bind to the RCP, the fluorescence decreases. Therefore, lower fluorescence is indicative of greater miRNA concentrations.

References:

Kaur, A., Mackin, S. T., Schlosser, K., Wong, F. L., Elharram, M., Delles, C., Stewart, D. J., Dayan, N., Landry, T., & Pilote, L. (2020). Systematic review of microRNA biomarkers in acute coronary syndrome and stable coronary artery disease. Cardiovascular research, 116(6), 1113–1124. https://doi.org/10.1093/cvr/cvz302
Zhang, R., Lan, C., Pei, H., Duan, G., Huang, L., & Li, L. (2015). Expression of circulating miR-486 and miR-150 in patients with acute myocardial infarction. BMC cardiovascular disorders, 15, 51. https://doi.org/10.1186/s12872-015-0042-0

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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-This part is the sequence for hsa-miR-150-5p, an miRNA isolated from <i> Homo sapiens </i>. This miRNA acts as a biomarker for coronary artery disease and is therefore potentially useful for the early detection of this condition. hsa-miR-150-5p is related to the inflammation process and is involved in the pathogenesis of coronary artery disease. Therefore, hsa-miR-150-5p is a means by which cardiologists can monitor the progression of the disease in patients (Zhang et al., 2015). However, hsa-miR-150-5p seems to be dysregulated, increased, and decreased in concentration. Nevertheless, more research, with larger sample sets is needed to see if this biomarker is viable for CAD diagnosis (Kaur et al., 2020).
+This part is the sequence for hsa-miR-150-5p, an miRNA isolated from <i> Homo sapiens </i>. This miRNA acts as a biomarker for coronary artery disease and is therefore potentially useful for the early detection of this condition. hsa-miR-150-5p is related to the inflammation process and is involved in the pathogenesis of coronary artery disease. Therefore, hsa-miR-150-5p is a means by which cardiologists can monitor the progression of the disease in patients (Zhang et al., 2015). However, hsa-miR-150-5p seems to be dysregulated, increased and decreased in concentration. Nevertheless, more research, with larger sample sets is needed to see if this biomarker is viable for CAD diagnosis (Kaur et al., 2020).
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