Difference between revisions of "Part:BBa K4245009"

Revision as of 00:01, 11 October 2022

hsa-mir-133a-3p

This part is the sequence for hsa-miR-133a-3p, an miRNA isolated from Homo sapiens . This miRNA acts as an upregulated biomarker for coronary artery disease (Kaur et al., 2020), and is therefore potentially useful for the early detection of this condition. Similar to BBa_K4245006, hsa-miR-133a-3p, or miRNA-133a-3p, is seen to increase in blood circulation potentially hours and days before a myocardial infarction, even before Troponin T is increased in circulation, and it is also related with cardiac-specific muscle lineage (Xiao et al., 2019). Thus, both biomarkers increase the sensitivity of our biosensors, as both miRNA-1-3p and miRNA-133a-3p in higher concentrations could indicate a possible heart attack.

The Lambert_GA 2022 team developed a set of padlock probes to use the rolling circle amplification approaches for several miRNAs related to CAD. This miRNA is used as the basis for BBa_K4245103 and BBa_K4245110, the 3' arm for hsa-miR-133a-3p and 5' arm for hsa-miR-133a-3p, and as the target sequence for BBa_K4245204 and BBa_K4245205 the hsa-miR-133a-3p RCA Padlock Probe and the hsa-miR-133a-3p RCT Padlock Probe.

When using rolling circle amplification (RCA), the miRNA binds to the padlock. A rolling circle product (RCP) is produced from BBa_K4245131 (Middle Sequence), which is then detected by the linear probes BBa_K4245130 (Fluorophore) and BBa_K4245132 (Quencher). When these parts bind to the RCP, the fluorescence decreases. Therefore, lower fluorescence is indicative of greater miRNA concentrations.

References:

Kaur, A., Mackin, S. T., Schlosser, K., Wong, F. L., Elharram, M., Delles, C., Stewart, D. J., Dayan, N., Landry, T., & Pilote, L. (2020). Systematic review of microRNA biomarkers in acute coronary syndrome and stable coronary artery disease. Cardiovascular research, 116(6), 1113–1124. https://doi.org/10.1093/cvr/cvz302
Xiao, Y., Zhao, J., Tuazon, J. P., Borlongan, C. V., & Yu, G. (2019). MicroRNA-133a and Myocardial Infarction. Cell transplantation, 28(7), 831–838. https://doi.org/10.1177/0963689719843806

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K4245009 short</partinfo>
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-This part is the sequence for hsa-miR-133a-3p, an miRNA isolated from <i> Homo sapiens </i>. This miRNA acts as an upregulated biomarker for coronary artery disease (Kaur et al., 2020), and is therefore potentially useful for the early detection of this condition. Similar to <partinfo>BBa_K4245006</partinfo>, hsa-miR-133a-3p, or miRNA-133a-3p, is seen to increase in blood circulation potentially hours and days before a myocardial infarction, even before Troponin T is increased in circulation, as it is also related with cardiac-specific muscle lineage (Xiao et al., 2019). Thus, both biomarkers increase the sensitivity of our biosensors, as both miRNA-1-3p and miRNA-133a-3p in higher concentration could indicate a possible heart attack.
+This part is the sequence for hsa-miR-133a-3p, an miRNA isolated from <i> Homo sapiens </i>. This miRNA acts as an upregulated biomarker for coronary artery disease (Kaur et al., 2020), and is therefore potentially useful for the early detection of this condition. Similar to <partinfo>BBa_K4245006</partinfo>, hsa-miR-133a-3p, or miRNA-133a-3p, is seen to increase in blood circulation potentially hours and days before a myocardial infarction, even before Troponin T is increased in circulation, and it is also related with cardiac-specific muscle lineage (Xiao et al., 2019). Thus, both biomarkers increase the sensitivity of our biosensors, as both miRNA-1-3p and miRNA-133a-3p in higher concentrations could indicate a possible heart attack.
 <br>
 <br>
-The Lambert_GA 2022 team developed a set of padlock probes to be used with the rolling circle amplification approaches for several miRNAs related to CAD. This miRNA is used as the basis for <partinfo>BBa_K4245103</partinfo> and <partinfo>BBa_K4245110</partinfo>, the 3' arm for hsa-miR-133a-3p and 5' arm for hsa-miR-133a-3p, and as the target sequence for <partinfo>BBa_K4245204</partinfo>  and  <partinfo>BBa_K4245205</partinfo>  the hsa-miR-133a-3p RCA Padlock Probe and the hsa-miR-133a-3p RCT Padlock Probe.
+The Lambert_GA 2022 team developed a set of padlock probes to use the rolling circle amplification approaches for several miRNAs related to CAD. This miRNA is used as the basis for <partinfo>BBa_K4245103</partinfo> and <partinfo>BBa_K4245110</partinfo>, the 3' arm for hsa-miR-133a-3p and 5' arm for hsa-miR-133a-3p, and as the target sequence for <partinfo>BBa_K4245204</partinfo>  and  <partinfo>BBa_K4245205</partinfo>  the hsa-miR-133a-3p RCA Padlock Probe and the hsa-miR-133a-3p RCT Padlock Probe.
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