Difference between revisions of "Part:BBa K4164018"

Revision as of 17:42, 10 October 2022

Contribution

This composite part is used to do further research on the yellow fluorescent protein (YFP), which is commonly used as the reporter of a gene circuit. We chose the Bobrick BBa_K592101 from the iGEM distribution kit and recombined it to plasmid pET-29a+ by homologous recombination. We firstly studied the effect of different concentration of IPTG(Isopropyl-beta-D-thiogalactopyranoside) on the expression of YFP and the fluorescence intensity of bacteria. After that, we also extracted the YFP from the bacteria and identified the effect of temperature on YFP activity.

Figure1.YFP fluorescence intensity after 30 mins incubation under different temperatures.

Figure2. Effect of different IPTG concentrations on YFP expression

Figure3. 4 hours YFP-transformed E. coli strains (Right: control)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 741

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 We chose the Bobrick BBa_K592101 from the iGEM distribution kit and recombined it to plasmid pET-29a+ by homologous recombination.
 We firstly studied the effect of different concentration of IPTG(Isopropyl-beta-D-thiogalactopyranoside) on the expression of YFP and the fluorescence intensity of bacteria. After that, we also extracted the YFP from the bacteria and identified the effect of temperature on YFP activity.
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 <img src="https://static.igem.wiki/teams/4164/wiki/part-registry/part-018-1.png"with="700" height="" width="350" height=""/>