Difference between revisions of "Part:BBa K4335004"

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	Our optimized mCherry for <i>Chlamydomonas reinhardtii</i>.In addition to the optimization of codes, we also added a intron length of 145 bp .<br>		Our optimized mCherry for <i>Chlamydomonas reinhardtii</i>.In addition to the optimization of codes, we also added a intron length of 145 bp .<br>
	<br>The existence of introns in eukaryotes is an important characteristic that distinguishes them from prokaryotes. In higher organisms, introns have been reported to regulate gene expression at multiple levels. The main function of introns is to produce different exonic combinations through alternative splicing and then translate different proteins, thus increasing the complexity of the proteome. [2]<br>		<br>The existence of introns in eukaryotes is an important characteristic that distinguishes them from prokaryotes. In higher organisms, introns have been reported to regulate gene expression at multiple levels. The main function of introns is to produce different exonic combinations through alternative splicing and then translate different proteins, thus increasing the complexity of the proteome. [2]<br>
−	<br>As a eukaryote, <i>Chlamydomonas reinhardtii</i> can express mCherry gene by adding promoter.	+	<br>As a eukaryote, <i>Chlamydomonas reinhardtii</i> can express mCherry gene by adding intron.
		+	<figure>
		+	<img src="https://static.igem.wiki/teams/4335/wiki/mcherry-3.png" width="100%" style="float:center">
		+	<figcaption>
		+	<p style="font-size:1rem">The Top Blue Band is BBa_K2136016, the Middle Red Band is mCherry's coding sequence, and the bottom band is mCherry with introns.
		+	</p>
		+	</figcaption>
		+	</figure>
		+	<h2>Result</h2>
		+
		+
		+	</html>
	<!-- Add more about the biology of this part here		<!-- Add more about the biology of this part here
	===Usage and Biology===		===Usage and Biology===

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Revision as of 16:53, 10 October 2022

mCherry

mCherry is a red fluorescent protein. Fluorescent reporter genes for biological applications. We optimized its codon to express in Chlamydomonas reinhardtii more efficiently.

BBa_K4335004 is the DNA of mCherry gene derived from mushroom coral and it is codon optimized for Chlamydomonas reinhardtii .

The advantage of mcherry over other fluorescent proteins is that its color and green fluorescent protein (GFP) are co-labeled, and mcherry also has excellent light stability over other monomeric fluorescent proteins.

It's believed that codon-choice have been conserved during evolution course and because not all tRNA are expressed equally, specially across species, a particular DNA sequence can be codon optimised to match the most prevalent tRNAs of the host cell, improving the efficiency of protein translation [1].

When we looked through the blast tool on the registry page, we found a part [BBa_K2136016] that was very similar to ours.

This is also a codon optimized mCherry for Chlamydomonas reinhardtii.

We compared the two sequences using snapgene and found that they were identical to our mcherry coding sequence.

Our optimized mCherry for Chlamydomonas reinhardtii.In addition to the optimization of codes, we also added a intron length of 145 bp .

The existence of introns in eukaryotes is an important characteristic that distinguishes them from prokaryotes. In higher organisms, introns have been reported to regulate gene expression at multiple levels. The main function of introns is to produce different exonic combinations through alternative splicing and then translate different proteins, thus increasing the complexity of the proteome. [2]

As a eukaryote, Chlamydomonas reinhardtii can express mCherry gene by adding intron.

Result

Sequence and Features