Difference between revisions of "Part:BBa K4375009"
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==Usage and Biology== | ==Usage and Biology== | ||
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The GS linker is a flexible linker that can connect different protein domains or regions. This stretch is rich in serine and glycine amino acids and thus provides high mobility to the protein regions conjugated to it. GS linkers are usually useful in connecting antibody fragments to achieve better antigen binding or improve protein folding. | The GS linker is a flexible linker that can connect different protein domains or regions. This stretch is rich in serine and glycine amino acids and thus provides high mobility to the protein regions conjugated to it. GS linkers are usually useful in connecting antibody fragments to achieve better antigen binding or improve protein folding. | ||
The BamHI site makes it easy to clone the linker sequence. This makes it convenient for usage in molecular cloning. | The BamHI site makes it easy to clone the linker sequence. This makes it convenient for usage in molecular cloning. | ||
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==References== | ==References== | ||
| − | + | Chen, X.; Zaro, J. L.; Shen, W.-C. Fusion Protein Linkers: Property, Design and Functionality. Advanced Drug Delivery Reviews 2013, 65 (10), 1357–1369. https://doi.org/10.1016/j.addr.2012.09.039. | |
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Latest revision as of 16:18, 10 October 2022
GS linker with BamHI restriction site
A 13 amino acid long GS linker (Glycine-Serine linker) with a BamHI restriction site at its C-terminal.
Usage and Biology
The GS linker is a flexible linker that can connect different protein domains or regions. This stretch is rich in serine and glycine amino acids and thus provides high mobility to the protein regions conjugated to it. GS linkers are usually useful in connecting antibody fragments to achieve better antigen binding or improve protein folding. The BamHI site makes it easy to clone the linker sequence. This makes it convenient for usage in molecular cloning.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 28
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Chen, X.; Zaro, J. L.; Shen, W.-C. Fusion Protein Linkers: Property, Design and Functionality. Advanced Drug Delivery Reviews 2013, 65 (10), 1357–1369. https://doi.org/10.1016/j.addr.2012.09.039.