Difference between revisions of "Part:BBa K2205000"
Svetlana ia (Talk | contribs) |
Svetlana ia (Talk | contribs) |
||
Line 8: | Line 8: | ||
Due to time constraints, work on assembling and further characterising this Biobrick was unable to finish. | Due to time constraints, work on assembling and further characterising this Biobrick was unable to finish. | ||
− | + | <html><a href="https://2022.igem.wiki/tu-dresden/">TU_Dresden 22 team</a></html> suggests to use this part could use as an <b>alternative</b> to <html><a href="https://parts.igem.org/Part:BBa_J04450">BBa_J04450</a></html> for white-red colony selection. | |
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 15:19, 10 October 2022
J23100-RFP
BBa_K2205000 is a proposed composite BioBrick reporter that constitutively produces MFRP1. It possesses an MRFP1 coding sequence (E1010) after a strong Anderson promoter (J23100) and RBS (B0034). A double terminator (B0015) was added after the MRFP1 sequence. A BioBrick RFC10 prefix and suffix were added as well.
The construct was submitted to IDT for synthesis as a gBlock. Due to time constraints, work on assembling and further characterising this Biobrick was unable to finish.
TU_Dresden 22 team suggests to use this part could use as an alternative to BBa_J04450 for white-red colony selection.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 616
Illegal AgeI site found at 728 - 1000COMPATIBLE WITH RFC[1000]
Part Documentation by TU_Dresden 2022 Team
Table of content |
---|
|
- Biology
- Expression
- Comparison to constitutative promoter
- Item4
Selection of constitutive promotor
Comparison with BBa_J04450
Part was compared to BBa_J04450.
Usage and Biology
TEst