Difference between revisions of "Part:BBa K2205000"
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# Comparison to constitutative promoter | # Comparison to constitutative promoter | ||
# Item4 | # Item4 | ||
| + | ==Selection of constitutive promotor== | ||
| + | ==Comparison with <html><a href="https://parts.igem.org/Part:BBa_J04450">BBa_J04450</a></html>== | ||
Part was compared to <html><a href="https://parts.igem.org/Part:BBa_J04450">BBa_J04450</a></html>. | Part was compared to <html><a href="https://parts.igem.org/Part:BBa_J04450">BBa_J04450</a></html>. | ||
[[File:BBa_K2205000_fig1_comparison_with_LAC.png|thumb|center|600px| | [[File:BBa_K2205000_fig1_comparison_with_LAC.png|thumb|center|600px| | ||
Revision as of 15:14, 10 October 2022
J23100-RFP
BBa_K2205000 is a proposed composite BioBrick reporter that constitutively produces MFRP1. It possesses an MRFP1 coding sequence (E1010) after a strong Anderson promoter (J23100) and RBS (B0034). A double terminator (B0015) was added after the MRFP1 sequence. A BioBrick RFC10 prefix and suffix were added as well.
The construct was submitted to IDT for synthesis as a gBlock. Due to time constraints, work on assembling and further characterising this Biobrick was unable to finish.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 616
Illegal AgeI site found at 728 - 1000COMPATIBLE WITH RFC[1000]
Part Documentation by TU_Dresden 2022 Team
| Table of content |
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- Biology
- Expression
- Comparison to constitutative promoter
- Item4
Selection of constitutive promotor
Comparison with BBa_J04450
Part was compared to BBa_J04450.
Figure 1: A. Lac promoter of BBa_J04450 part and mRFP1 reporter downstream in presence of inductor (IPTG) and in its absence. B. Fluorescence/absorbance 600nm of mRFP1 of the BBa_J04450 compared to BBa_K2205000 part with J23100 promoter over 24 hour incubation of bacterial culture.
Usage and Biology
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