Difference between revisions of "Part:BBa K4245210"

Latest revision as of 16:40, 13 October 2022

T7 regulated miRNA1-broccoli-spacer
This part is the T7 regulated miRNA1-broccoli-spacer, designed to test the efficacy of the hsa-miR-1-3p RCT Padlock Probe (BBa_K4245201). We were unsure if the inclusion of a spacer sequence (BBa_K4245160) affected the folding of the broccoli aptamer (BBa_K3380153). The part was designed to imitate the transcript produced when Rolling Circle Transcription (RCT) is applied to hsa-miR-1-3p RCT Padlock Probe.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 137
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4245210 short</partinfo>
 <br>
-This part is the T7 regulated miRNA1-broccoli-spacer, designed to test the efficacy of our part, <partinfo>BBa_K4245201</partinfo>, the hsa-miR-1-3p RCT Padlock Probe. We were unsure if the inclusion of a spacer sequence (<partinfo>BBa_K4245160</partinfo>) affected the folding of the broccoli aptamer (<partinfo>BBa_K3380153</partinfo>). The part was designed to imitate the transcript produced when Rolling Circle Transcription (RCT) is applied to hsa-miR-1-3p RCT Padlock Probe.
+This part is the T7 regulated miRNA1-broccoli-spacer, designed to test the efficacy of the hsa-miR-1-3p RCT Padlock Probe (<partinfo>BBa_K4245201</partinfo>). We were unsure if the inclusion of a spacer sequence (<partinfo>BBa_K4245160</partinfo>) affected the folding of the broccoli aptamer (<partinfo>BBa_K3380153</partinfo>). The part was designed to imitate the transcript produced when Rolling Circle Transcription (RCT) is applied to hsa-miR-1-3p RCT Padlock Probe.
 <!-- Add more about the biology of this part here