Difference between revisions of "Part:BBa K4195119"
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Agarose Gel Electrophoresis When we were constructing this circuit, regular PCR was used to certify the plasmid was correct. We got the target (2360 bp). | Agarose Gel Electrophoresis When we were constructing this circuit, regular PCR was used to certify the plasmid was correct. We got the target (2360 bp). | ||
| + | [[File:T--XMU-China--BBa K4195119.png|200px]] | ||
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| + | '''<b>Fig 1. The result of colony PCR. Plasmid pSB1C3. </b>''' | ||
Revision as of 14:34, 10 October 2022
I0500-B0034-GFP-B0015
Biology and Usage
I0500 is the inducible pBad/araC promoter. The promoter of the araBAD operon from E. coli is activated in the presence of arabinose and the absence of glucose and directs expression of genes encoding enzymes required for arabinose metabolism. gfp can express green fluorescence protein. The expression level of GFP can be used to characterize the function of I0500.
Characterization
Agarose Gel Electrophoresis When we were constructing this circuit, regular PCR was used to certify the plasmid was correct. We got the target (2360 bp).
Fig 1. The result of colony PCR. Plasmid pSB1C3.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1880
Illegal SapI site found at 961