Difference between revisions of "Part:BBa K4165201"
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the sequence was optimized for <i>E.coli</i> expression | the sequence was optimized for <i>E.coli</i> expression | ||
<partinfo>BBa_K4165201 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4165201 SequenceAndFeatures</partinfo> | ||
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+ | ===Dry Lab=== | ||
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+ | <p style=" font-weight: bold; font-size:14px;"> Modeling </p> | ||
+ | tTrim21-GSGS-Coh2 is modeled by AlphaFold2, ITASSER, Robetta and TrRosetta, best model obtained from TrRosetta. | ||
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+ | <html> | ||
+ | <p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/switches/trim-gsgs-coh2.png" style="margin-left:150px;" alt="" width="500" /></p> | ||
+ | </html> | ||
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+ | Figure 1.: Predicted 3D structure of our fusion protein tTrim21-GSGS-Coh2. | ||
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+ | <p style=" font-weight: bold; font-size:13px;"> Table 1: Quality assessment parameters of tTrim21-GSGS-Coh2. model. </p> | ||
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+ | <html> | ||
+ | <p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/switches/trim-gsgs-coh2qa.png" style="margin-left:75px;" alt="" width="700" | ||
+ | /></p> | ||
+ | </html> | ||
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===References=== | ===References=== |
Revision as of 20:28, 11 October 2022
Trim-(GSGS)-Coh2
This parts code for the trim21 E3 ligase having his PRYSPRY domain truncated (BBa_K3396007), fused to type 1 Cohesin module derived from Clostridium thermocellum cellulosome scaffoldin using Glycine serine flexible linker repeated two times to maintain part flexibility needed during target ubiquitination
Source
Synthetic Fusion protein
Sequence and Features
the sequence was optimized for E.coli expression
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 225
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 182
- 1000COMPATIBLE WITH RFC[1000]
Dry Lab
Modeling
tTrim21-GSGS-Coh2 is modeled by AlphaFold2, ITASSER, Robetta and TrRosetta, best model obtained from TrRosetta.
Figure 1.: Predicted 3D structure of our fusion protein tTrim21-GSGS-Coh2.
Table 1: Quality assessment parameters of tTrim21-GSGS-Coh2. model.
References
1- Carvalho AL, Dias FM, Nagy T, Prates JA, Proctor MR, Smith N, Bayer EA, Davies GJ, Ferreira LM, Romão MJ, Fontes CM, Gilbert HJ. Evidence for a dual binding mode of dockerin modules to cohesins. Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3089-94. doi: 10.1073/pnas.0611173104. Epub 2007 Feb 20. PMID: 17360613; PMCID: PMC1805526.