Difference between revisions of "Part:BBa K190015:Experience"

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The part (<partinfo>BBa_K190015</partinfo>) was used in our overall arsenic clean up E.coli to activate gas vesicle production upon arsenic detection. The promoter seemed to activate formation of vesicles without addition of arsenic in our tests. Either the ArsR regulator protein is produced in a too low amount to control unwanted expression (in witch case the gene for ArsR has to be upregulated), or the arsenic levels in our water are already sufficient for activation of expression.
 
The part (<partinfo>BBa_K190015</partinfo>) was used in our overall arsenic clean up E.coli to activate gas vesicle production upon arsenic detection. The promoter seemed to activate formation of vesicles without addition of arsenic in our tests. Either the ArsR regulator protein is produced in a too low amount to control unwanted expression (in witch case the gene for ArsR has to be upregulated), or the arsenic levels in our water are already sufficient for activation of expression.
 
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<partinfo>BBa_K190033 AddReview 2</partinfo>
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<I>iGEM Groningen 2009</I>
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Relative promoter units were calculated from RFP fluorescence upon arsenite induction of the ArsR-promoter. The promoter had an induction of 2.26 (RPU) after induction for 3hrs with 100uM NaAsO2. This is an internal concentration between 0.8-1.2uM As (as seen in Figure 1).
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[[Image:RFP over As conc.PNG|Figure 1: RFP fluorescence increasing upon RFP expression under pArsR promoter (K190015)]]
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<!-- DON'T DELETE --><partinfo>BBa_K190015 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K190015 EndReviews</partinfo>

Revision as of 18:50, 20 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K190015

User Reviews

UNIQ5ef2775e4e57a5e0-partinfo-00000000-QINU

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iGEM Groningen 2009

The part (BBa_K190015) was used in our overall arsenic clean up E.coli to activate gas vesicle production upon arsenic detection. The promoter seemed to activate formation of vesicles without addition of arsenic in our tests. Either the ArsR regulator protein is produced in a too low amount to control unwanted expression (in witch case the gene for ArsR has to be upregulated), or the arsenic levels in our water are already sufficient for activation of expression.

;
••

iGEM Groningen 2009

Relative promoter units were calculated from RFP fluorescence upon arsenite induction of the ArsR-promoter. The promoter had an induction of 2.26 (RPU) after induction for 3hrs with 100uM NaAsO2. This is an internal concentration between 0.8-1.2uM As (as seen in Figure 1). Figure 1: RFP fluorescence increasing upon RFP expression under pArsR promoter (K190015)

;

UNIQ5ef2775e4e57a5e0-partinfo-00000004-QINU