Difference between revisions of "Part:BBa K209473:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K209473 short</partinfo>
 
<partinfo>BBa_K209473 short</partinfo>
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===Source===
 
===Source===
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Parent plasmid from Invitrogen.
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===References===
 
===References===

Latest revision as of 21:19, 21 October 2009

pcDNA3.1(+) with AarI A-D sites in MCS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 1065
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2387
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 952
    Illegal NheI site found at 895
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2387
    Illegal NotI site found at 978
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 952
    Illegal BglII site found at 12
    Illegal BamHI site found at 929
    Illegal XhoI site found at 1059
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 1065
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2387
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 1065
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2387
    Illegal NgoMIV site found at 1497
    Illegal NgoMIV site found at 2838
    Illegal NgoMIV site found at 3121
    Illegal AgeI site found at 1013
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 882
    Illegal BsaI.rc site found at 4646
    Illegal SapI site found at 3566
    Illegal SapI.rc site found at 2687
    Illegal SapI.rc site found at 2897


Design Notes

Source

Parent plasmid from Invitrogen.

References