Difference between revisions of "Part:BBa K4165116"
(→‎Modeling)
Line 19: Line 19:
 
===Modeling===
 
===Modeling===
 
<html>
 
<html>
−
<p><img src="" style="margin-left:200px;" alt="" width="500" /></p>
+
<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1v.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
</html>
 
</html>
  

Revision as of 17:08, 10 October 2022


HTRA Binding Peptide 22

part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein

Usage and Biology

Synthetic peptide acquired by Phage display, Binds specifically to the water-sites of PDZ domain of HTRA1 Protein with IC50 of 0.16 ± 0.01 and ΔG of 8.0 kJ mol-1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

Got score of 4 out of 6 in our Quality assessment code after modeling by AlphaFold2.0

cbeta_deviations = 0, clashscore = 170.45, molprobity = 4.83, ramachandran_favored = 0, ramachandran_outliers = 66.67, Qmean_4 = -6.45183, Qmean6 = -5.51397

Modeling


                 Figure 1.: 3D Model of H1V peptide Modelled by AlphaFold2.0 displayed in Pyomol

References

Beuming, T., Farid, R., & Sherman, W. (2009). High‐energy water sites determine peptide binding affinity and specificity of PDZ domains. Protein Science, 18(8), 1609-1619.