Difference between revisions of "Part:BBa K2935068"
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==Contribution From NNU-China 2022== | ==Contribution From NNU-China 2022== | ||
| − | '''Group''': [https://2022.igem. | + | '''Group''': [https://2022.igem.wiki/nnu-china/] |
'''Author''': Xiaolu Sun | '''Author''': Xiaolu Sun | ||
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===Characterization from iGEM22-NNU-China=== | ===Characterization from iGEM22-NNU-China=== | ||
| + | ===IDH2 Gene=== | ||
Isocitrate dehydrogenase (IDH) is a key enzyme in the TCA cycle, which can provide reducing power for cell growth, and participate in algal energy metabolism and anabolism[1]. IDH2 is the gene encoded in Y.lipolytica. It was registered in 2019. In order to characterize the effect of the IDH2 gene in lipid synthesis and growth, we constructed a knockout strain po1f ΔylIDH2 using homologous recombination. The YNB medium have been chosen for testing Results showed that the strain po1f ΔylIDH2 can effectively increase the accumulation of biomass and fatty acid content. Among them, the strain po1f ΔylIDH2 biomass and fatty acid content increased by 5.63% and 10.10%, respectively. These results provide references for future iGEM teams to improve biomass and lipid accumulation in Y.lipolytica. | Isocitrate dehydrogenase (IDH) is a key enzyme in the TCA cycle, which can provide reducing power for cell growth, and participate in algal energy metabolism and anabolism[1]. IDH2 is the gene encoded in Y.lipolytica. It was registered in 2019. In order to characterize the effect of the IDH2 gene in lipid synthesis and growth, we constructed a knockout strain po1f ΔylIDH2 using homologous recombination. The YNB medium have been chosen for testing Results showed that the strain po1f ΔylIDH2 can effectively increase the accumulation of biomass and fatty acid content. Among them, the strain po1f ΔylIDH2 biomass and fatty acid content increased by 5.63% and 10.10%, respectively. These results provide references for future iGEM teams to improve biomass and lipid accumulation in Y.lipolytica. | ||
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Revision as of 13:27, 10 October 2022
IDH2
Isocitrate dehydrogenase(IDH) is a key enzyme in the TCA cycle, IDH2 is it’s gene, which can provide reducing power for cell growth, and participate in algal energy metabolism and anabolism.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 718
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 718
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 143
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 718
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 718
Illegal NgoMIV site found at 778 - 1000COMPATIBLE WITH RFC[1000]
Contribution From NNU-China 2022
Group: [1]
Author: Xiaolu Sun
Characterization from iGEM22-NNU-China
IDH2 Gene
Isocitrate dehydrogenase (IDH) is a key enzyme in the TCA cycle, which can provide reducing power for cell growth, and participate in algal energy metabolism and anabolism[1]. IDH2 is the gene encoded in Y.lipolytica. It was registered in 2019. In order to characterize the effect of the IDH2 gene in lipid synthesis and growth, we constructed a knockout strain po1f ΔylIDH2 using homologous recombination. The YNB medium have been chosen for testing Results showed that the strain po1f ΔylIDH2 can effectively increase the accumulation of biomass and fatty acid content. Among them, the strain po1f ΔylIDH2 biomass and fatty acid content increased by 5.63% and 10.10%, respectively. These results provide references for future iGEM teams to improve biomass and lipid accumulation in Y.lipolytica.
- Fig. 1. The Growth and lipid synthesis of strain po1f ΔylIDH2 under different culture conditions. A. Changes of growth curves in two fermentation mediums. B. Changes of fatty acid content in the two fermentation mediums at 120h
Reference
[1] Morgunov, I. G., Solodovnikova, N. Y., Sharyshev, A. A., Kamzolova, S. V., & Finogenova, T. V. (2004). Regulation of NAD+-Dependent isocitrate dehydrogenase in the citrate producing yeast Yarrowia lipolytica. Biochemistry (Moscow), 69(12), 1391-1398.