Difference between revisions of "Part:BBa K4375003"
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This promoter was used to regulate BLADE protein transcription in ELTE 2022 group’s project. It was important that this promoter by the literature is weaker than J23101 Anderson promoter because in this case, the production of the protein means less metabolic stress for the bacteria.
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This promoter was used to regulate BLADE protein transcription in ELTE 2022 group’s project, which was obtained from the work of Romano et. al, who also used to produce BLADE in E. coli. It was important that this promoter by the literature is weaker than the wt J23101 Anderson promoter because, in this case, the production of the protein means less metabolic stress for the bacteria.
  
 
This promoter is shorter than the J23101. The nucleotide with the red frame is missing from it. This change leads to a weaker promoter.
 
This promoter is shorter than the J23101. The nucleotide with the red frame is missing from it. This change leads to a weaker promoter.
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Latest revision as of 19:12, 10 October 2022


Modified Constitutive Promoter (J23101)

J23101* is part of the J23, Anderson constitutive promoter family, it is the modified version J23101 Anderson promoter (BBa_J23101). It is weaker than the original promoter.



Usage and Biology

Figure 1: The nucleotide in the red frame is missing one from J23101*.

This promoter was used to regulate BLADE protein transcription in ELTE 2022 group’s project, which was obtained from the work of Romano et. al, who also used to produce BLADE in E. coli. It was important that this promoter by the literature is weaker than the wt J23101 Anderson promoter because, in this case, the production of the protein means less metabolic stress for the bacteria. This promoter is shorter than the J23101. The nucleotide with the red frame is missing from it. This change leads to a weaker promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 29
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

Romano, E.; Baumschlager, A.; Akmeriç, E. B.; Palanisamy, N.; Houmani, M.; Schmidt, G.; Öztürk, M. A.; Ernst, L.; Khammash, M.; Di Ventura, B. Engineering AraC to Make It Responsive to Light Instead of Arabinose. Nature Chemical Biology 2021, 17 (7), 817–827. https://doi.org/10.1038/s41589-021-00787-6.