Difference between revisions of "Part:BBa K4325024"
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<partinfo>BBa_K4325024 short</partinfo>
 
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<h4>
 
<h4>
 
1.Batch screening of pDawn(without LVA tag)-X174E-T1 in response to blue light lysis in <i> E. coli</i>.</h4>
 
1.Batch screening of pDawn(without LVA tag)-X174E-T1 in response to blue light lysis in <i> E. coli</i>.</h4>
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[[File:2222222222222222.png|600px|thumb|center|Figure1. (1)pSEVA331-pDawn(cI-LVA)-X174E-LVA-T1; (2)pSEVA331-pDawn(cI)-X174E-T1; (3)pSEVA331-pDawn(cI-LVA)-RBS070-LKD-LVA-T1; (4)pSEVA331-pDawn(cI)-RBS070-LKD-T1 were constructed successfully.]]<P></P> [[File:3333333.png|600px|thumb|center|Figure2.  Growth condition of pSEVA331-pDawn(cI)-X174E-T1-TOP10 in dark and under light.]]<p></p> As shown in Figure 1, we successfully constructed pSEVA331-pDawn(cI)-X174E-T1 and transformed it into <i> E. coli</i> TOP10, selection a monoclonal colony and culturing in different growth conditions. As shown in Figure 2, the 9th colony of pSEVA331-pDawn(cI)-X174E-T1-TOP10 grew in the dark, but did not grow under blue light.
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[[File:K24 1.png|600px|thumb|center|Figure1. (1)pSEVA331-pDawn(cI-LVA)-X174E-LVA-T1; (2)pSEVA331-pDawn(cI)-X174E-T1; (3)pSEVA331-pDawn(cI-LVA)-RBS070-LKD-LVA-T1; (4)pSEVA331-pDawn(cI)-RBS070-LKD-T1 were constructed successfully.]]<P></P> [[File:K24 2.png|600px|thumb|center|Figure2.  Growth condition of pSEVA331-pDawn(cI)-X174E-T1-TOP10 in dark and under light.]]<p></p> As shown in Figure 1, we successfully constructed pSEVA331-pDawn(cI)-X174E-T1 and transformed it into <i> E. coli</i> TOP10, selection a monoclonal colony and culturing in different growth conditions. As shown in Figure 2, the 9<sup>th</sup> colony of pSEVA331-pDawn(cI)-X174E-T1-TOP10 grew in the dark, but did not grow under blue light.
 
<h3>References</h3>
 
<h3>References</h3>
 
<p>Robert Ohlendorf 1 , Roee R. Vidavski 2 , Avigdor Eldar 2, Keith Moffat 3,4 and Andreas Möglich 1, 3.From Dusk till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression.
 
<p>Robert Ohlendorf 1 , Roee R. Vidavski 2 , Avigdor Eldar 2, Keith Moffat 3,4 and Andreas Möglich 1, 3.From Dusk till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression.
 
</p >
 
</p >

Revision as of 13:18, 11 October 2022


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Description

This composite part is a generator consisting of pDawn (without LVA tag) (Status: 500 Content-type: text/html

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) and X174E (Status: 500 Content-type: text/html

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).

Usage

We inserted the pDawn (without LVA tag) blue light response system (Status: 500 Content-type: text/html

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)with the lysis gene X174E (Status: 500 Content-type: text/html

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)into the pSEVA331 expression vector, which was inserted into E. coli TOP10. The bacterial colony that grew in the dark but did not grow under blue light were screened to verify the responsiveness of pDawn (without LVA tag) to blue light.


Sequence and Features

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2022 SZPT-China

Characterization

1.Batch screening of pDawn(without LVA tag)-X174E-T1 in response to blue light lysis in E. coli.

Figure1. (1)pSEVA331-pDawn(cI-LVA)-X174E-LVA-T1; (2)pSEVA331-pDawn(cI)-X174E-T1; (3)pSEVA331-pDawn(cI-LVA)-RBS070-LKD-LVA-T1; (4)pSEVA331-pDawn(cI)-RBS070-LKD-T1 were constructed successfully.
<P>

Figure2. Growth condition of pSEVA331-pDawn(cI)-X174E-T1-TOP10 in dark and under light.

As shown in Figure 1, we successfully constructed pSEVA331-pDawn(cI)-X174E-T1 and transformed it into E. coli TOP10, selection a monoclonal colony and culturing in different growth conditions. As shown in Figure 2, the 9th colony of pSEVA331-pDawn(cI)-X174E-T1-TOP10 grew in the dark, but did not grow under blue light.

References

Robert Ohlendorf 1 , Roee R. Vidavski 2 , Avigdor Eldar 2, Keith Moffat 3,4 and Andreas Möglich 1, 3.From Dusk till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression.