Difference between revisions of "Part:BBa K4182000:Design"

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Neurospora crassa，sequence found through NCBI(https://www.ncbi.nlm.nih.gov/gene/3873728)
 
Neurospora crassa，sequence found through NCBI(https://www.ncbi.nlm.nih.gov/gene/3873728)
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===References===
 
===References===

Revision as of 12:02, 10 October 2022


VVDH


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 223
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 223
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 223
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 223
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Performed E. coli codon optimization


Source

Neurospora crassa，sequence found through NCBI(https://www.ncbi.nlm.nih.gov/gene/3873728)

References

[1] ROMANO E, BAUMSCHLAGER A, AKMERIÇ E B, et al. Engineering AraC to make it responsive to light instead of arabinose [J]. Nat Chem Biol, 2021, 17(7): 817-27.

[2] RAMAKRISHNAN P, TABOR J J. Repurposing Synechocystis PCC6803 UirS-UirR as a UV-Violet/Green Photoreversible Transcriptional Regulatory Tool in E. coli [J]. ACS Synth Biol, 2016, 5(7): 733-40.