Difference between revisions of "Part:BBa K4225008:Design"

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===Source===
 
===Source===
  
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All parts except TEV protease are obtained from biobricks. For the source of TEV protease, refer to the basic part BBa_K4225009.
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All parts except TEV protease are obtained from biobricks. For the source of TEV protease, refer to the basic part BBa_K4225009. Prefix-Suffix are added to our sequence and spacers are also added between the parts. This composite part is also codon optimized for our chassis, DH5α.
  
 
===References===
 
===References===

Latest revision as of 14:37, 12 October 2022


TEV protease induced by Ptac and cMyc-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 855
    Illegal XhoI site found at 165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 439
    Illegal SapI.rc site found at 787


Design Notes

Source

All parts except TEV protease are obtained from biobricks. For the source of TEV protease, refer to the basic part BBa_K4225009. Prefix-Suffix are added to our sequence and spacers are also added between the parts. This composite part is also codon optimized for our chassis, DH5α.

References