Difference between revisions of "Part:BBa K215210"
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This part should display WT streptavidin + 6His, BBa_J36841, on the surface of the cell via the OmpA display system when induced with IPTG.
 
This part should display WT streptavidin + 6His, BBa_J36841, on the surface of the cell via the OmpA display system when induced with IPTG.
  
−
Note: in order to express this construct as a fusion protein, there's no T after this sequence and before the SpeI site.
+
Note: in order to express this construct as a fusion protein, there is no T after this sequence and before the SpeI site.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:57, 1 October 2009

IPTG Inducible 1tmr OmpA Surface Display of WT Streptavidin

This part should display WT streptavidin + 6His, BBa_J36841, on the surface of the cell via the OmpA display system when induced with IPTG.

Note: in order to express this construct as a fusion protein, there is no T after this sequence and before the SpeI site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 337
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 379
    Illegal AgeI site found at 430
  • 1000
    COMPATIBLE WITH RFC[1000]